General descriptionCell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Also available as a 5 mg/ml mM solution in H2O (Cat. No. 508741).Fluorescent dye specific for DNA. Binds preferentially to DNA rich in adenine and thymidine (excitation max. = 359 nm, emission max. = 461 nm). Useful for microscopic analysis of nuclei and nuclear DNA in apoptotic cells. Can also be used to detect mycoplasma. Reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.Biochem/physiol ActionsCell permeable: noPrimary TargetBinds preferentially to DNA rich in adenine and thymineProduct does not compete with ATP.Reversible: yesWarningToxicity: Standard Handling (A)ReconstitutionFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 6 weeks at 4°C or for up to 6 months at -20°C.Other NotesMathieu, C., et al. 1995. Exp. Cell Res.216, 13.Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105, 251.Hotz, M.A., et al. 1994. Cytometry15, 237.Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods 26, 249.Miller, T., et al. 1993. Biotechniques15, 1042.Gray, J.W., et al. 1982. Science238, 323.Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.Russels, W.C., et al. 1975. Nature253, 461.Legal InformationCALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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