Anti-Sphingosine Kinase 1 (Ser-225), Phosphospecific Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: SPHK1
Host: Rabbit
Rrid: AB_2195826
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: Sphingosine Kinase 1 (Ser-225)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: SK1, SPHK1, SPK1
Clonality: Rabbit Polyclonal
Immunogen: Phospho-SK1 (Ser-225) synthetic peptide (coupled to carrier protein) corresponding to amino acids surrounding serine 225 in human SK1. This sequence has 4 amino acid differences from mouse and 5 from rat SK1, and is not homologous to sequences in SK2. The conserved site in rat and mouse SK1 is at serine 224.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: This antibody was cross-adsorbed to non-specific phosphoserine and unphosphorylated SK1 (Ser-225) peptide before affinity purification using phospho-SK1 (Ser-225) peptide. The purified antibody detects a 47 kDa* full-length recombinant human SK1 protein phosphorylated with ERK2.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 42
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human
Target Description: Sphingolipids are metabolized into bioactive products that include ceramide, sphingosine, and sphingosine-1-phosphate (S1P). Sphingosine Kinase (SK) catalyzes the phosphorylation of the lipid sphingosine, creating S1P. S1P subsequently signals through cell surface G protein-coupled receptors, as well as intracellularly, to modulate cell proliferation, survival, motility and differentiation. Two isoforms of SK have been identified, SK1 and SK2. The mRNA for both of these isoforms is widely expressed with SK1 expression highest in brain, heart, kidney, thymus, spleen and lung, while SK2 is highest in kidney and liver. SKs can be activated through growth factor, G protein-coupled, and immunoglobulin receptor signalling. SK1 has been shown to mediate cell growth, prevention of apoptosis, and cellular transformation, and is upregulated in a variety of human tumors. Regulation of SK1 may occur through ERK mediated phosphorylation of Ser-225. This phosphorylation leads to increased activity and translocation to the plasma membrane.
Uniprot Number Immunogen Species: Q9NYA1
Post Translational Modification Type: Phosphorylated
Post Translational Modification Residue: Ser-225