ACE2 Lentivirus

DESCRIPTION

Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found on the surface of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the two strains that caused outbreaks of Severe acute respiratory syndrome (SARS-CoV) and coronavirus disease 2019 (COVID-19) (SARS-CoV-2). The ACE2 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an ACE2 gene (NM_021804.3) driven by an EF1a promoter.

DETAILS

Genbank: NM_021804.3
Shiptemp: -80°C (dry ice)
Synonyms: Angiotensin converting enzyme 2, ACEH, SARS-CoV, Coronavirus, COVID-19, SARS-CoV-2, ACE2 cells, ACE2 cell assay, covid19
Warnings: Biosafety: None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions.
Category: Coronavirus/Lentivirus
Description: Human Angiotensin converting enzyme 2 (ACE2), also known as ACEH, is an integral membrane protein found on the surface of cells in the lungs, arteries, heart, kidney, and intestines. ACE2 serves as the entry point into cells for some coronaviruses, including the two strains that caused outbreaks of Severe acute respiratory syndrome (SARS-CoV) and coronavirus disease 2019 (COVID-19) (SARS-CoV-2). The ACE2 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an ACE2 gene (NM_021804.3) driven by an EF1a promoter.
Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
Supplied As: Two vials (500 µl x 2) of lentivirus at a titer ≥1 x 10^7 TU/ml. The titer will vary with each lot; the exact value is provided with each shipment.
Unspsc Code: 41106621
Unspsc Name: Virus mediated expression vectors or kits
Applications: 1. Transient expression of ACE2 in target cells.2. Generation of a stable cell line expressing ACE2 with Puromycin selection.
Product Type: Lentivirus
Biosafety Level: BSL-2
Related Products: 100679, 79942, 79943, 79959
Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
Scientific Category: Coronavirus