DESCRIPTION

• Form: Lyophilized powder
• Host: Rabbit
• Clone: NA
• Label: ATTO-594. Maximum absorption 601 nm; Maximum fluorescence 627 nm. The fluorescence is excited most efficiently in the 580 - 615 nm range. This label belongs to the class of Rhodamine dyes and can be used with fluorescent equipment typically optimized to detect Texas Red and Alexa-594.
• Purity: Affinity purified on immobilized antigen.
• Target: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: 25103
• Isotype: Rabbit IgG
• Homology: Mouse - identical; human - 13/14 amino acid residues identical
• Is Toxin: No
• Sequence: CYEGVNILRIPAKR, corresponding to amino acid residues 95-108 of rat nAChRβ4
• Synonyms: nAChR β4, Neuronal acetylcholine receptor subunit beta-4, Cholinergic receptor nicotinic beta 4
• Accession: P12392
• Clonality: Polyclonal
• Lead Time: 1-2 Business Days
• Reactivity: Human|Rat|Mouse
• Formulation: PBS pH7.4, 1% BSA with 0.05% sodium azide
• Applications: IC|IF|IHC|LCI
• Preservative: 1% BSA, 0.05% NaN3
• Reconstitution: 50 µl double distilled water (DDW).
• Blocking Peptide: BLP-NC014
• Negative Control: BLP-NC014
• Positive Control: NA
• Cited Application: ICC
• Immunogen Location: Extracellular, N-terminus
• Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
• Shipping and Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
• Immunogen Source Species: Rat
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C, protected from the light, for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
• Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
• Applications May Also Work In: IC|IF|IHC|LCI
• Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
• Product Page - Scientific Background: Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs); muscarinic AChRs (mAChRs), which belong to the G-protein coupled receptor (GPCR) superfamily, and nicotinic AChRs (nAChRs), which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which are responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, three conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer of these various subunits organized around a central pore1.While most β subunits are neuronal, the β1 subunit forms functional receptors along with other subunits in the muscle3. In neurons the α2-α6 and β2-β4 subunits form heteropentameric receptors, usually with a (αx)2(βy)3 stoichiometry3.
• Standard Quality Control of Each Lot: Western blot analysis (unlabeled antibody, #ANC-014), and immunohistochemistry (labeled antibody).
• Application Dilutions Western Blot Wb: Contact Alomone for information
• Antibody Concentration After Reconstitution: 1 mg/ml
• Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:100