DESCRIPTION

• Category: REAGENTS
• Keywords: flow cytometry antibody, flow cytometry antibodies, facs antibody, primary antibody, monoclonal antibody, anti-human antibody, Anti human, anti human antibody anti-CD16, CD16, anti CD16, 3G8, Clone 3G8, conjugated antibody, conjugated flow cytometry antibody, CD16 antibody, CD Marker, CD Markers, CD Antigen, APC-Cyanine7 Antibody, APC-Cyanine7 CD16
• Paragraph 1: The clone 3G8, a mouse monoclonal antibody, binds to the 50-65 kDa transmembrane protein of IgG Fc?RIII known as CD16, a surface antigen commonly associated with human NK cells. In addition to surface expression on NK cells, CD16 is expressed on activated monocytes, macrophages, neutrophils, and placental trophoblasts in humans. 3G8 binds either with the aggregated IgG or IgG-antigen complex which functions in NK cell activation, phagocytosis, and antibody-dependent cell mediated cytotoxicity (ADCC). Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.
• Paragraph 2: Each lot of these antibodies has been pre-titrated and tested by flow cytometric analysis of human PBMCs such that 0.5µg (unconjugated, Biotin) or 5µl (conjugated) of these products are sufficient for staining 1 million cells in a 100µl staining volume or 100µl of whole blood. It is recommended to titrate antibody reactivity empirically for optimal performance. Non-human primate cross-reactivity has been validated using the APC conjugated clone 3G8 product. Do you need an antibody for an antigen you don't see on our website? Do you need a different conjugation than what we have listed? We have a wide selection of antigens with a variety of conjugations available. Please reach out to us at support@geneseesci.com.
• Bullet Point 1: Monoclonal antibody specific to human and non-human primate CD16
• Bullet Point 2: The 3G8 antibody has been reported for use in flow cytometric analysis and functional assays
• Bullet Point 3: Highly specific and little to no cross reactivity to ensure accurate, reproducible results in Flow Cytometry
• Bullet Point 4: >90% pure tested via polyacrylamide gel electrophoresis (PAGE)
• Bullet Point 5: More conjugations and unit sizes available. Contact us at support@geneseesci.com to inquire.
• Manufacturer Sku: 101495