DESCRIPTION

• Form: Antigen Affinity Purified from Pooled Serum
• Gene: ATRIP
• Host: Rabbit
• Rrid: AB_2492046
• Type: Primary Antibody
• Buffer: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
• Target: ATRIP (Ser239)
• Isotype: IgG
• Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
• Shipping: Blue Ice
• Synonyms: AGS1 antibody, ATIP antibody, ATM and Rad3 related interacting protein antibody, ATM and Rad3-related-interacting protein antibody, ATR interacting protein antibody, ATR-interacting protein antibody, Atrip antibody, ATRIP_HUMAN antibody, DKFZp762J2115 antibody, FLJ12343 antibody, MGC20625 antibody, MGC21482 antibody, MGC26740 antibody
• Clonality: Polyclonal
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser239 of human ATRIP, conjugated to keyhole limpet hemocyanin (KLH).
• Expiration: After date of receipt, stable for at least 1 year at -20°C.
• Specificity: Specific for endogenous levels of the ~86 kDa ATRIP protein phosphorylated at Ser239. The antibody also recognizes a band at ~32 kDa.
• Applications: WB
• Ncbi Gene Id: 84126
• Conjugate Exem: Unconjugated
• Physical State: Liquid
• Quality Control: Western blots performed on each lot.
• Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
• Molecular Weight: 86
• Production Notes: Prepared from rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.
• Dilution Range Wb: 1:500
• Immunogen Species: Human
• Species Reactivity: Mouse
• Target Description: ATRIP, ATR interacting protein, binds to ATR to regulate ATR expression, and is an essential component of the DNA damage checkpoint pathway (Cortez et al, 2001). ATR is recruited to DNA lesions in part through its association with ATRIP, which in turn interacts with the single-stranded DNA binding protein RPA (Ball et al, 2007). DNA replication forks may stall as a result of DNA damage causing phosphorylation of several proteins, including BRCA1 when colocalizing with ATR/ATRIP complex and RPA (Venere et al, 2007). The DNA replication fork stall coincides with BRCA1 directly phosphorylating ATRIP at Ser-239 (Venere et al, 2007).
• Expected Reactivity: Bovine, Human, Non-Human Primate, Sheep
• Uniprot Number Immunogen Species: Q8WXE1
• Post Translational Modification Type: Phosphorylated
• Post Translational Modification Residue: Ser239

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