DESCRIPTION

• Aa: 2-end (all components)
• Mw: 325 kDa (complex)
• Tags: N-terminal His-tag (EZH1, SUZ12, AEBP2, RbAp48), N-terminal FLAG-tag (EED)
• Format: Aqueous buffer solution
• Genbank: NM_001991 (EZH1), NM_003797(EED), NM_015355 (SUZ12), NM_153207(AEBP2), NM_005610(RbAP48)
• Species: Human
• Uniprot: Q92800
• Shiptemp: -80°C (dry ice)
• Synonyms: KMT6B, EZH, Retinoblastoma Binding Protein 4 (RBBP4)
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Enzymes
• References: 1. Margueron R. et al. Mol. Cell 32, 4, 503-518, 2008. 2. Shen X. et al., Mol. Cell 32, 4, 491-502, 2008.
• Description: Complex of human EZH1, GenBank Accession No. NM_001991, a.a. 2-end, with N-terminal His-tag, MW= 86 kDa, human EED, NM_003797, a.a. 2-end, with N-terminal FLAG-tag, MW= 51 kDa, human SUZ12, NM_015355, a.a. 2- end, with N-terminal His-tag, MW = 87 kDa, human AEBP2, NM_153207, a.a. 2-end, with N-terminal His-tag, MW= 53 kDa, and human RbAp48, NM_005610, a.a. 2-end, with N-terminal His-tag, MW = 48 kDa, co-expressed in baculovirus expression system.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 200 mM imidazole, and 20% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: insect
• Product Type: Protein
• Host Cell Line: Sf9 cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 50 µl reaction mix (20 mM phosphate pH 7.4, 0.05% Tween-20, 40 µM S-adenosylmethionine, and enzyme complex) add to the wells coated with the substrate on a Neutravidin white plate (G-Biosciences). Incubate for 1 hr. Add antibody against methylated K27 residue of histone H3, incubate 1 hr. Then, add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminescence.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Methyltransferase