CR-ACT-3-66737
Mouse 4921534H16Rik Transomic CRISPR target gene set for gene activation (gRNA+dCas9-VPR)
Transomic
DESCRIPTION
Transomic's all-in-one lentiviral vectors for gene activation incorporate a gRNA targeting the gene of interest and the inactive dCas9-VPR complex, eliminating the need for transducing cell lines with multiple vectors. All gRNAs are designed by Transomic and incorporate features of the CRoatan algorithm (https://www.cell.com/molecular-cell/fulltext/S1097-2765(17)30464-1) and consider data from ENCODE (https://www.encodeproject.org/) to ensure gRNAs target open DNA tracks for efficient gene activation. Each target gene set includes 3 different constructs targeting the gene of interest and a negative control. Choose from glycerol stock and ready-to-use lentiviral particle formats (100ul of 1x10^6 TU/ml per construct).
DETAILS
- Gene: 4921534H16Rik
- Gene Id: 66737
- Markers: Blasticidin, Puromycin, ZsGreen
- Species: Mouse
- Category: CRISPR
- Application: Gene Activation
- Vector Name: pACT-ALL