Monoclonal Antibody to Bcl-X (Apoptosis Marker)(Clone : SPM165)

DESCRIPTION

Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.

DETAILS

Gene: BCL2L1
App Id: FACS,IF,WB,IHC
Antigen: Bcl-X (Apoptosis Marker)
Gene Id: 598
Isotype: Mouse IgG2a, kappa
Format Id: Purified
Clone Name: SPM165
Uniprot Id: Q07817
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Affinity Chromatography
Reactivity Id: Human, Mouse, Rat
Product Content: 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Alternative Name: BCL2L1||BCL2L||BCLX
Application Note: Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95&degC followed by cooling at RT for 20 minutes)
Storage Condition: Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Immunogen Information: A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein