DESCRIPTION

• Aa: 25-167
• Mw: 44.5 kDa
• Tags: C-terminal Fc fusion (Human IgG1), C-terminal Avi-tag™
• Notes: HiP™ indicates a high purity protein with ≥90% purity as measured by gel filtration. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
• Format: Aqueous buffer solution
• Genbank: NM_005018
• Species: Human
• Uniprot: Q15116
• Shiptemp: -80°C (dry ice)
• Synonyms: Programmed cell death 1, PDCD1, PD-1, PD1, SLEB2, CD279, HPD-L
• Warnings: Avoid freeze/thaw cycles. Protein may be diluted to ≥100 µg/ml in PBS + glycerol and stored at -80°C.
• Category: Immunotherapy/Protein
• References: 1. Shinohara, T., et al., Genomics. 1994 Oct;23(3):704-6.2. James, E.S., et al., Genes Immun. 2005 Aug;6(5):430-7.
• Description: Human secreted programmed cell death 1 (PD-1)-Fc fusion protein, also known as, PDCD1, SLEB2, CD279, HPD-L, PD-1 Biotin-labeled GenBank Accession No. NM_005018, a.a 25-167, fused at the C-terminus to the Fc portion of human IgG1, with C-terminal Avitag ™ expressed in a HEK293 cell expression system and enzymatically biotinlabeled using Avi-tag™ technology. MW = 44.5 kDa. This protein runs at a higher M.W. by SDS-PAGE due to glycosylation.
• Formulation: 8 mM phosphate, pH 7.4, 110 mM NaCl, 2.2 mM KCl, and 20% glycerol
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of protein-protein interaction and in vitro and in vivo protein function.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: BPS Cat. 72005 protocol was followed. Briefly, 50 µl of 2 µg/ml PD-L1 was coated onto plates. 20 µl of 0.5 µg/ml PD-1, Biotin-labeled was allowed to bind at room temp. for 2 hours. Strep-HRP was added and allowed to incubate at room temp for 1 hr, followed by HPR chemiluminescent substrate addition and plate reading.
• Related Products: 79523, 71106, 79512, 79670
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Immunotherapy