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CFS-FLEX-MS

FLEXIQuant PLUS Expression Kit

CellFree Sciences

DESCRIPTION

Protein synthesis kit with 13C/15N labeled lysine and arginine for MRM/SRM mass spectrometry. Kit uses bilayer reaction on 226 μl scale. This kit provided expression vector and peptide to conduct FLEXIQuant experiments for using the FLEX-tag for quantifying the reference standard.

DETAILS

  • Features: Kit enables direct production of reference standards for the FLEXIQuant method 13C and 15N isotopes-labeled lysine and arginine included into reaction buffer suitable for working with trypsin digestion in bottom-up proteomics Very high labeling efficiency with incorporation rates of over 99% Special expression and control vector included to utilize FLEX-tag for absolute protein quantification by FLEXIQuant method All reagents included to directly prepare labeled proteins from DNA template Premixed reagents to synthesize proteins with just a few pipetting steps Kit contains reagents for 8 translation reactions on 226 µl reaction scale
  • Tag Line: Labeled protein standards for mass spectrometry and proteomics
  • Applications: Preparation of stable-isotope-labeled reference standards for protein mass spectrometry. Working with the FLEX-tag allows for easy quantification of the reference protein.
  • Storage Condition:  -80 ℃