SEK12045
Human IkB alpha / NFKBIA ELISA Pair Set
Sino Biological
Image
DESCRIPTION
The Human IkB alpha / NFKBIA ELISA Pair Set is for the quantitative determination of Human IkB alpha / NFKBIA.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IkB alpha / NFKBIA coated on a 96-well plate. Standards and samples are added to the wells, and any IkB alpha / NFKBIA present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated mouse anti-IkB alpha / NFKBIA monoclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of IkB alpha / NFKBIA present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 22 ng of recombinant NFKBIA. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 0.8 ng/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 12.5-800
- Specificity: Human IkB alpha / NFKBIA
- Capture Antibody: 1.0 mg/mL of rabbit anti-NFKBIA monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in CBS before coating. (Catalog: # 12045-R056)
- Detection Antibody: 0.5 mg/mL mouse anti-NFKBIA monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % glycerol, pH 7.4). Dilute to working concentration of 0.5 μg/mL in detection antibody dilution buffer before use. (Catalog: # 12045-MM02)