SPR-494B SPR-494C SPR-494E
Tau-352 (0N3R) and Alpha Synuclein Co-Polymer Fibrils
Stressmarq Biosciences
Image
![](https://www.stressmarq.com/wp-content/uploads/SPR-494_Tau-and-Alpha-Synuclein-co-polymer-fibrils-Protein-TEM-1.png)
Image
![](https://www.stressmarq.com/wp-content/uploads/SPR-494_Tau-and-Alpha-Synuclein-co-polymer-fibrils-Protein-Immuno-TEM-1.png)
Image
![](https://www.stressmarq.com/wp-content/uploads/SPR-494_Tau-and-Alpha-Synuclein-co-polymer-fibrils-Protein-SDS-Page-1.png)
Image
![](https://www.stressmarq.com/wp-content/uploads/SPR-494_Tau-and-Alpha-Synuclein-co-polymer-fibrils-Protein-Thioflavin-T-assay-1.png)
DESCRIPTION
Human Recombinant Tau-352 (fetal 0N3R) and Human Recombinant Alpha Synuclein Co-Polymer Fibrils
DETAILS
- Nature: Recombinant
- Purity: >95%
- Target: Tau and Alpha Synuclein Co-Polymer
- Category: Protein
- Conjugate: No Tag
- References: 1. Goedert et al. Multiple Isoforms of Human Microtubule-associated Protein Tau: Sequences and Localization in Neurofibrilary Tangles of Alzheimer’s Disease. Neuron. 1989;3(4):519-526. 2. Jensen et al. α-synuclein Binds to Tau and Stimulates the Protein Kinase A-catalyzed Tau Phosphorylation of Serine Residues 262 and 356. 1999. JBC. 274(36): 25481-25489. DOI:https://doi.org/10.1074/jbc.274.36.25481 3. Giasson et al. Initiation and Synergistic Fibrillization of Tau and Alpha-Synuclein. Science. 2003; 300: 636-40. DOI: 10.1126/science.1082324 4. Guo et al. Distinct α-synuclein Strains Differentially Promote Tau Inclusions in Neurons. 2013. Cell. 154(1) doi:10.1016/j.cell.2013.05.057. 5. Williams et al. Differential Cross-seeding Properties of Tau and α-synuclein in Mouse Models of Tauopathy and Synucleinopathy. Brain Communications. 2020; 2(2):fcaa090. doi:10.1093/braincomms/fcaa090 6. Pan et al. Tau Accelerates α-synuclein Aggregation and Spreading in Parkinson’s Disease. 2022. Brain. Doi: 10.1093/brain/awac172
- Applications: WB | SDS PAGE | In vitro assay
- Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
- Protein Size: 0N3R: 36.76 kDa, ASYN: 14.46 kDa
- Purification: Ion-exchange Purified
- Concentration: Total Protein Concentration: 2mg/mL (1mg/ml of tau and 1mg/ml of aSyn)
- Protein Length: Full Length (Tau 0N3R: 1-352 aa, Asyn: 1-140 aa)
- Research Areas: Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy
- Storage Buffer: 1X PBS pH 7.4
- Alternative Names: MAPT, Fetal Tau, 0N3R, neurofibrillary tangle protein, paired-helical filament, PHFs, SNCA, NACP, PARK1, asyn, alpha-synuclein, pre-formed fibril, PFFs, mixed fibrils
- Cite This Product: Human Recombinant Tau and Alpha Synuclein Co-Polymer Fibrils (StressMarq Biosciences Inc. | Victoria BC CANADA | Catalog # SPR-494B)
- Expression System: E. coli
- Species Full Name: Human
- Amino Acid Sequence: Tau: MAEPRQEFEVMEDHAGTYGLGDRKDQGGYTMHQDQEGDTDAGLKAEEAGIGDTPSLEDEAAGHVTQARMVSKSKDGTGSDDKKAKGADGKTKIATPRGAAPPGQKGQANATRIPAKTPPAPKTPPSSGEPPKSGDRSGYSSPGSPGTPGSRSRTPSLPTPPTREPKKVAVVRTPPKSPSSAKSRLQTAPVPMPDLKNVKSKIGSTENLKHQPGGGKVQIVYKPVDLSKVTSKCGSLGNIHHKPGGGQVEVKSEKLDFKDRVQSKIGSLDNITHVPGGGNKKIETHKLTFRENAKAKTDHGAEIVYKSPVVSGDTSPRHLSNVSSTGSIDMVDSPQLATLADEVSASLAKQGL Asyn: MDVFMKGLSKAKEGVVAAAEKTKQGVAEAAGKTKEGVLYVGSKTKEGVVHGVATVAEKTKEQVTNVGGAVVTGVTAVAQKTVEGAGSIAAATGFVKKDQLGKNEEGAPQEGILEDMPVDPDNEAYEMPSEEGYQDYEPEA
- Storage Temperature: -80ºC
- Shipping Temperature: Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
- Scientific Background: Brain-specific tau isoforms vary in the number of N-terminal inserts and C- terminal repeat domains due to alternative splicing of exons; only the shortest isoform of tau, 0N3R, is expressed in the fetal brain during neurogenesis (1). Tau and alpha-synuclein polymerize into amyloid fibrils to form filamentous inclusions in neurodegenerative diseases such as Alzheimer’s and Parkinson’s disease. Tau has been shown to interact with alpha-synuclein in vitro (2), with synergistic cross-seeding between tau and alpha-synuclein resulting in polymerization of each other into fibrillary amyloid lesions in neuronal cultures and in vivo (3,4). Recombinant tau and alpha-synuclein co-polymer fibrils have demonstrated a more widespread transmission of induced pathology in a rodent model of tauopathies compared to pure Tau or alpha-synuclein fibrils alone (5). These co-polymer fibrils have also shown enhanced alpha-synuclein aggregation in vitro, and more severe alpha-synuclein pathology and Parkinson’s disease-like symptoms in mice (6).
- Certificate of Analysis: Protein certified >95% pure on SDS-PAGE & Nanodrop analysis