80551
PARP1 Chemiluminescent Assay Kit
BPS Bioscience
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DESCRIPTION
The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.
DETAILS
- Notes: This kit is also suitable for use with cell extracts, but nonspecific ribosylation activity may be detected.
- Shiptemp: -80°C (dry ice)
- Synonyms: poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1
- Warnings: Avoid freeze/thaw cycles.
- Category: PARP/Assay Kit
- Background: PARP1 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
- References: Brown, J.A., Marala, R.B., J. Pharmacol. Toxicol. Methods. 2002;47:137-41. Application Reference(s): 1. Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis (2013) 2. Structural basis and SAR for G007-LK, a lead stage 1,2,4-triazole based specific tankyrase 1/2 inhibitor (2013)
- Description: The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: The PARP1 assay kit comes in a convenient 96-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 100 enzyme reactions.
- Unspsc Code: 41105332
- Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
- Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 80560, 80580, 80584, 78317
- Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
- Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: PARP
- Instructions for Use: See assay kit datasheet for detailed protocol.