ARHGAP8 (NM_001017526) Human Tagged ORF Clone

DESCRIPTION:

ARHGAP8 (Myc-DDK-tagged)-Human Rho GTPase activating protein 8 (ARHGAP8), transcript variant 1

DETAILS

Mw: 53.3 kDa
Tag: Myc-DDK
Accn: NM_001017526
Type: Human Tagged ORF Clone
Refseq: NM_001017526.1, NP_001017526.1
Status: In Stock*
Symbol: ARHGAP8
Vector: pCMV6-Entry
Category: cDNA Clones
Locus Id: 23779
Orf Size: 1392 bp
Synonyms: BPGAP1; PP610
Refseq Orf: 1395 bp
Uniprot Id: P85298
Refseq Size: 1725 bp
Availability: In Stock
Cytogenetics: 22q13.31
Gene Summary: This gene encodes a member of the RHOGAP family. GAP (GTPase-activating) family proteins participate in signaling pathways that regulate cell processes involved in cytoskeletal changes. GAP proteins alternate between an active (GTP-bound) and inactive (GDP-bound) state based on the GTP:GDP ratio in the cell. This family member is a multidomain protein that functions to promote Erk activation and cell motility. Alternative splicing results in multiple transcript variants. Read-through transcripts from the upstream proline rich 5, renal (PRR5) gene into this gene also exist, which led to the original description of PRR5 and ARHGAP8 being a single gene. [provided by RefSeq, Nov 2010]
Chromatograms: CHROMATOGRAMS Sequencher program is needed, download here.
Sequence Data: ORF Nucleotide Sequence ( show hide ) >RC220168 representing NM_001017526 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCTGGCCAGGATCCTGCGCTGAGCACGAGTCACCCGTTCTACGACGTGGCCAGACATGGCATTCTGC AGGTGGCAGGGGATGACCGCTTTGGAAGACGTGTTGTCACGTTCAGCTGCTGCCGGATGCCACCCTCCCA CGAGCTGGACCACCAGCGGCTGCTGGAGTATTTGAAGTACACACTGGACCAATACGTTGAGAACGATTAT ACCATCGTCTATTTCCACTACGGGCTGAACAGCCGGAACAAGCCTTCCCTGGGCTGGCTCCAGAGCGCAT ACAAGGAGTTCGATAGGAAAGACGGGGATCTCACTATGTGGCCCAGGCTGGTCTCGAACTCCAAGCTCAA GCGATCCTCCCACCTCAGCCTCCCAAAGTACTGGGATTACAGGTACAAGAAGAACTTGAAGGCCCTCTAC GTGGTGCACCCCACCAGCTTCATCAAGGTCCTGTGGAACATCTTGAAGCCCCTCATCAGTCACAAGTTTG GGAAGAAAGTCATCTATTTCAACTACCTGAGTGAGCTCCACGAACACCTTAAATACGACCAGCTGGTCAT CCCTCCCGAAGTTTTGCGGTACGATGAGAAGCTCCAGAGCCTGCACGAGGGCCGGACGCCGCCTCCCACC AAGACACCACCGCCGCGGCCCCCGCTGCCCACACAGCAGTTTGGCGTCAGTCTGCAATACCTCAAAGACA AAAATCAAGGCGAACTCATCCCCCCTGTGCTGAGGTTCACAGTGACGTACCTGAGAGAGAAAGGCCTGCG CACCGAGGGCCTGTTCCGGAGATCCGCCAGCGTGCAGACCGTCCGCGAGATCCAGAGGCTCTACAACCAA GGGAAGCCCGTGAACTTTGACGACTACGGGGACATTCACATCCCTGCCGTGATCCTGAAGACCTTCCTGC GAGAGCTGCCCCAGCCGCTTCTGACCTTCCAGGCCTACGAGCAGATTCTCGGGATCACCTGTGTGGAGAG CAGCCTGCGTGTCACTGGCTGCCGCCAGATCTTACGGAGCCTCCCAGAGCACAACTACGTCGTCCTCCGC TACCTCATGGGCTTCCTGCATGCGGTGTCCCGGGAGAGCATCTTCAACAAAATGAACAGCTCTAACCTGG CCTGTGTCTTCGGGCTGAATTTGATCTGGCCATCCCAGGGGGTCTCCTCCCTGAGTGCCCTTGTGCCCCT GAACATGTTCACTGAACTGCTGATCGAGTACTATGAAAAGATCTTCAGCACCCCGGAGGCACCTGGGGAG CACGGCCTGGCACCATGGGAACAGGGGAGCAGGGCAGCCCCTTTGCAGGAGGCTGTGCCACGGACACAAG CCACGGGCCTCACCAAGCCTACCCTACCTCCGAGTCCCCTGATGGCAGCCAGAAGACGTCTC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RC220168 representing NM_001017526 Red =Cloning site Green =Tags(s) MAGQDPALSTSHPFYDVARHGILQVAGDDRFGRRVVTFSCCRMPPSHELDHQRLLEYLKYTLDQYVENDY TIVYFHYGLNSRNKPSLGWLQSAYKEFDRKDGDLTMWPRLVSNSKLKRSSHLSLPKYWDYRYKKNLKALY VVHPTSFIKVLWNILKPLISHKFGKKVIYFNYLSELHEHLKYDQLVIPPEVLRYDEKLQSLHEGRTPPPT KTPPPRPPLPTQQFGVSLQYLKDKNQGELIPPVLRFTVTYLREKGLRTEGLFRRSASVQTVREIQRLYNQ GKPVNFDDYGDIHIPAVILKTFLRELPQPLLTFQAYEQILGITCVESSLRVTGCRQILRSLPEHNYVVLR YLMGFLHAVSRESIFNKMNSSNLACVFGLNLIWPSQGVSSLSALVPLNMFTELLIEYYEKIFSTPEAPGE HGLAPWEQGSRAAPLQEAVPRTQATGLTKPTLPPSPLMAARRRL TR TRPLEQKLISEEDLAANDILDYKDDDDK V
Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
E Coli Selection: Kanamycin (25 ug/mL)
Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Mammalian Cell Selection: Neomycin