50157
FBXL10 (KDM2B) Chemiluminescent Assay Kit
BPS Bioscience
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DESCRIPTION
The FBXL10 Chemiluminescent Assay Kit is designed to measure FBXL10
activity for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, and
CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyllysine
4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10
Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate.
With this kit, only three simple steps on a microtiter plate are required for detection of
demethylase activity. First, FBXL10 enzyme is incubated with the methylated H3 peptide for one
hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary
antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be
measured using a chemiluminescence reader.
DETAILS
- Notes: The buffer in this kit was reformulated in May of 2015 with a different reducing agent to improve assay performance. The old formulation can still be purchased upon special request. MATERIALS REQUIRED BUT NOT SUPPLIED: TBST buffer (1 x TBS, pH 8.0, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Rotating or rocker platform
- Shiptemp: -80°C (dry ice)
- Synonyms: KDM2B, JHDM1B, CXXC2
- Warnings: Avoid freeze/thaw cycles.
- Category: Demethylase/Assay Kit
- References: Iwase, S., et al. Cell 2007; 128(6):1077-1088.
- Description: The FBXL10 Chemiluminescent Assay Kit is designed to measure FBXL10 activity for screening and profiling applications. FBXL10, also known as KDM2B, JHDM1B, and CXXC2, is a histone lysine demethylase that exhibits demethylation activity toward trimethyllysine 4 (H3K4me3) and dimethyl-lysine 36 (H3K36me2) on histone H3. The key to the FBXL10 Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for detection of demethylase activity. First, FBXL10 enzyme is incubated with the methylated H3 peptide for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by the addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: The FBXL10 Chemiluminescent Assay Kit comes in a convenient 96-well strip plate format, precoated with methylated histone H3 peptide substrate, primary antibody, secondary HRP-labeled antibody, demethylase assay buffer, and purified FBXL10 for 100 enzyme
- Unspsc Code: 41105331
- Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
- Applications: Great for studying enzyme kinetics and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 50120, 50610, 50156
- Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
- Storage Stability: One year from date of receipt when stored as directed.
- Scientific Category: Demethylase
- Instructions for Use: See product datasheet for detailed protocol.