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40589-V08H32

SARS-CoV-2 (BA.4/BA.5) Spike S1+S2 trimer Protein (ECD, His Tag)

Sino Biological

DESCRIPTION

A DNA sequence encoding the SARS-CoV-2 (BA.4) Spike S1+S2 (YP_009724390.1, with mutations F817P, A892P, A899P, A942P, K986P, V987P, T19I, L24del, P25del, P26del, A27S, H69del, V70del, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, L452R, S477N, T478K, E484A, F486V, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K and furin cleavage site mutants) expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant BA.4).

DETAILS

  • Tag: C-His
  • Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
  • Source: HEK293 Cells
  • Species: SARS-CoV-2
  • Storage: Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Activity: Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 (BA.4/BA.5/BA.5.2) Spike S1+S2 trimer Protein (ECD, His Tag)(Cat:40589-V08H32), the EC50 is 8-32 ng/mL.
  • Molecule: Spike
  • Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
  • Lead Time: 1-3 days
  • Formulation: Lyophilized from a sodium citrate buffer system at pH 6.0.
  • Molecular Mass: The recombinant SARS-CoV-2 (BA.4) Spike S1+S2 consists of 1226 amino acids and predicts a molecular mass of 136.26 kDa.
  • Reconstitution: A hardcopy of datasheet with reconstitution instructions is sent along with the products. Please refer to it for detailed information.
  • Accession Number: YP_009724390.1
  • Predicted N Terminal: Val 16