E0899Hu
Human Granzymes B, GZMS-B ELISA Kit
Bioassay Technology Laboratory
DESCRIPTION
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed: 3262682, PubMed: 3263427, PubMed: 1985927). It cleaves after Asp (PubMed: 8258716, PubMed: 1985927). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (PubMed: 32188940, PubMed: 31953257). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis (PubMed: 9852092).
DETAILS
- GeneID: 3002
- Species: Human
- Storage: 2-8ºC
- MASS(Da): 27,716
- Synonyms: C11;Cathepsin G-like 1;CTLA-1;CTSGL1;Cytotoxic T-lymphocyte proteinase 2;Fragmentin-2;Granzyme B;Granzyme-2;GZMB;HLP;Human lymphocyte protein;Lymphocyte protease;SECT;T-cell serine protease 1-3E
- Assay Time: 1h 30m
- Assay Type: Sandwich
- Gene Names: GZMB
- Reactivity: Human
- Sample Type: Serum, plasma, cell culture supernates
- Sensitivity: 0.22ng/L
- Target Protein: GZMB
- Detection Range: 0.5-300ng/L
- Full Product Name: Granzymes B
- Standard Solution: 320ng/L
- UniProt Accession: P10144