Anti-βIII-Tubulin (C-terminus) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: TUBB3
Host: Rabbit
Rrid: AB_2210672
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: βIII-Tubulin (C-terminus)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: TUBB3
Clonality: Rabbit Polyclonal
Immunogen: βIII-Tubulin synthetic peptide (coupled to KLH) corresponding to amino acid residues at the C-terminus of human βIII-Tubulin. This sequence is not found in bI or βII-Tubulin isotypes, but is well conserved in βIII-Tubulins from rat and mouse.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 50 kDa* protein corresponding to the molecular mass of βIII-Tubulin on SDS-PAGE immunoblots of purified brain tubulin and mouse brain tissue.
Applications: WB, ICC, IHC
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 50
Dilution Range Wb: 1:2000
Immunogen Species: Human
Dilution Range Icc: 1:200
Dilution Range Ihc: 1:500
Species Reactivity: Human, Mouse, and Rat
Target Description: Microtubules (MTs) are cytoskeletal elements that play an essential role in cell division and cytoplasmic organization. MTs are dynamic polymers of α/β-tubulin heterodimers. At least two populations of MTs, called dynamic and stable according to their rates of turnover, are readily distinguishable in cells. The proteins associated with MTs (MAPs) are among the best-known factors that regulate MT dynamics and stability. In addition, a variety of different post-translational modifications may also regulate MT dynamics and stability. Phosphorylation is one of these modifications and it can occur on serine, threonine, and tyrosine residues in β-Tubulin isoforms. Multiple kinases can phosphorylate Ser-444 at the C-terminus of βIII-Tubulin in vitro. Unphosphorylated Ser-444 in βIII-Tubulin is an early marker for cells of neuronal lineage, while phosphorylation of Ser-444 is upregulated after neuronal maturation and may preferentially occur in assembled MTs. By contrast, Cdk1 phosphorylation of Ser-172 in β-Tubulin occurs in mitotic cells and may impair tubulin incorporation into microtubules.
Uniprot Number Immunogen Species: Q13509