NALCN Antibody, Clone N187/7: ATTO 390

DESCRIPTION:

Mouse Anti-Rat NALCN Monoclonal IgG1

DETAILS

Target: NALCN
Gene Id: 266760
Isotype: IgG1
Conjugate: ATTO 390
Immunogen: Fusion protein amino acids 1659-1738 (cytoplasmic C-terminus) of rat NALCN
References: 1. Lee, J.H., Cribbs, L.L. and Perez-Reyes, E. (1999)FEBS Lett. 445: 231-236. 2. Lu, B., Su, Y., Das, S., Liu, J., Xia, J. and Ren, D. (2007) Cell 129: 371-383. 3. Jospin, M., et al. (2007) Curr. Biol. 17: 1595-1600. 4. Gaultier, C. and Gallego, J. (2008) Appl. Physiol. 104: 1522-1530. 5. Yeh, E., et al. (2008) PLoS Biol. 6: e55.
Swiss Prot: Q6Q760
Specificity: Detects ~200kDa.
Applications: WB | ICC/IF
Clone Number: N187/7 (Formerly sold as S187-7)
Field Of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
Host Species: Mouse
Product Type: Monoclonal
Purification: Protein G Purified
Concentration: 1 mg/ml
Research Areas: Neuroscience | Ion Channels | Sodium Channels | Sodium Leak Channels | Potassium Channels | Calcium Channels
Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
Accession Number: NP_705894.1
Alternative Names: VGCNL1 Antibody, CanIon Antibody, Sodium leak channel non-selective protein Antibody, Voltage gated channel-like protein 1 Antibody, A530023G15Rik Antibody, bA430M15.1 Antibody, FLJ23913 Antibody, FLJ44659 Antibody, FLJ44764 Antibody, Four repeat voltage gated ion channel Antibody, MGC74524 Antibody, Putative 4 repeat voltage gated ion channel Antibody, Sodium leak channel non-selective protein Antibody
Antibody Dilution: WB (1:1000), ICC/IF (1:100); optimal dilutions for assays should be determined by the user.
Cite This Product: StressMarq Biosciences Cat# SMC-417D-A390, RRID: AB_2701254
Immunogen Species: Rat
Storage Temperature: Conjugated antibodies should be stored according to the product label
Shipping Temperature: Blue Ice or 4ºC
Cellular Localization: Membrane
Scientific Background: NALCN (sodium leak channel non-selective protein), alsoknown as CanIon or VGCNL1 (voltage gated channel-like protein 1), is a 1738 amino acid multi-pass membrane protein that belongs to the cation-nonselective channel family. NALCN is highly conserved in mammals and is widely expressed in the central nervous system. Activated by NK-1R, NALCN is a voltage-independent, nonselective cation channel which is permeable to sodium, potassium and calcium ions. NALCN is responsible for background sodium ion leak conductance in neurons and regulates basal excitability of the nervous systems. Defects of NALCN in mice causes disruption in respiratory rhythm and death occurs within 24 hours of birth. Three isoforms of NALCN exists due to alternative splicing events.
Certificate Of Analysis: 1 µg/ml of SMC-417 was sufficient for detection of NALCN in 20 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.
Species Reactivity Full Name: Human | Mouse | Rat