DESCRIPTION

• Category: ZYMO KITS
• Keywords: D4076, D4077
• Paragraph 1: Quick-cfDNA™ Serum & Plasma Kit provides a simple and reliable method for the rapid preparation of high-quality circulating cell-free DNA from serum, plasma, amniotic fluid and cerebrospinal fluid (CSF). A combination of chemical and enzymatic methods are used to efficiently recover total DNA (including cell-free apoptotic, necrotic, mitochondrial and viral DNA) linearly from up to 10 ml of sample (Reference Figure A). Zymo-Spin™ technology allows for ultra-pure DNA to be eluted in as little as 35 µl water. The resulting DNA is suitable for all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses. Zymo’s serum and plasma cell-free DNA extraction technology will empower your discovery of circulating DNA biomarkers.
• Paragraph 2: Circulating cell-free DNA is found in various body fluids, including blood, and is generally derived from apoptotic cells, necrotic cells, and intact cells that were released into the bloodstream and eventually lysed [1-2]. Circulating cell-free DNA in serum and plasma is usually composed primarily of cell-free DNA fragments derived from healthy cells, however, in cancer patients circulating tumor DNA can be detected with a higher signal to noise ratio than whole blood for non-invasive diagnostics. Therefore, cell-free tumor DNA shed from circulating tumor cells is being investigated as a source of biomarkers for the early diagnosis, prognosis and monitoring of cancer therapy [3]. The utility and value of serum and plasma for non-invasive molecular diagnostics is demonstrated by the use of cell-free fetal DNA for sex determination and prenatal diagnosis of fetal chromosomal aneuploidy and other genetic disorders [4].
• Bullet Point 1: High-quality DNA, including cell-free, is easily and robustly purified from up to 10 ml of serum/plasma or up to 1 ml amniotic or cerebrospinal fluid
• Bullet Point 2: Zymo-Spin™ technology enables elution of DNA in as little as 35 µl and ensures it is ready for all sensitive downstream applications
• Bullet Point 3: Recover DNA >100bp (optimized for recovery of cell-free DNA)
• Bullet Point 4: Sample Sources: Serum, plasma, amniotic fluid, and cerebrospinal fluid (CSF).
• Bullet Point 5: Recovery Volume: as little as 35 µl of DNA Elution Buffer or DNase free water.
• Manufacturer Sku: D4076