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K21004

MagVigen™ - Protein G Kit

NVIGEN

DETAILS

  • Category: Purfication Kit
  • Lead Time: 1 week
  • Subcategory: Magnetic Beads
  • Applications: Protein Purification, Co-immunoprecipitation
  • Product and Related Product Contents: MagVigen™-Protein G nanoparticles are provided in phosphate buffered saline (PBS) containing 0.05% NaN3, 0.01% Tween 20. pH 7.4. Each vial contains 1 ml of solution with a particle concentration of 3 mg/ml, which is enough for approximately 300 μg of antibody enrichment.  Washing Buffer Elution Buffer All materials except Magnet should be stored at 4°C. Shelf life: 6 months

DESCRIPTION

Product Description MagVigen™ Protein G nanoparticles are ideal for antibody purification and immunoprecipitation assays. MagVigen™ Protein G nanoparticles recognize and efficiently bind to the Fc part of immunoglobulin (Ig) following a short incubation. The generated nanoparticle-antibody complex can be separated from the rest of the sample by magnet. The retained antibody can be eluted from the nanoparticles using an elution buffer. MagVigen™ nanoparticles enable identification of new protein-protein interactions through immunoprecipitation assays, where the MagVigen™ Protein G-antibody complex can be used to isolate particular proteins of interest or protein complex from assay samples, e.g. cell lysate. The immunoprecipitated proteins can be further analyzed by electrophoresis, protein staining, and mass spectrometry. MagVigen™ nanoparticles are much smaller than conventional micro-beads. This feature allows for better accessibility of the nanoparticles to the antigenic epitope and for less disturbance to the native functions of proteins or protein-protein complexes. In addition, the surfaces of MagVigen™ nanoparticles are uniquely coated to reduce non-specific interactions with cellular proteins and other biomolecules. This feature allows for a more specific “pull down” of real protein complex targets.