DESCRIPTION

• Target: Bassoon
• Gene Id: 8927
• Conjugate: FITC
• Immunogen: NM_003458.3 (AA 786-1041) N-terminal his-tagged fusion protein of human Bassoon
• References: 1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008–1029.
• Swiss-prot: Q9UPA5
• Specificity: Detects ~420kDa. Multiple isoforms can be detected.
• Applications: WB | IHC
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For in vitro research use only.
• Host Species: Rabbit
• Product Type: Polyclonal
• Purification: Protein A Purified
• Concentration: 1 mg/ml
• Research Areas: Neuroscience | Cell Markers | Neuron Markers | Presynaptic Markers | Neuroscience | Neurodegeneration | Multiple System Atrophy
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide *Storage buffer may change when conjugated
• Accession Number: NP_003449.2
• Alternative Names: BSN Antibody, ZNF231 Antibody, Neuronal double zine finger protein Antibody
• Antibody Dilution: WB (1:1000), IHC (1:400); optimal dilutions for assays should be determined by the user.
• Cite This Product: StressMarq Biosciences Cat# SPC-198D-FITC, RRID: AB_2704140
• Immunogen Species: Human
• Storage Temperature: Conjugated antibodies should be stored according to the product label
• Shipping Temperature: Blue Ice or 4ºC
• Cellular Localization: Cytoplasm | Cell Junction | Synapse
• Scientific Background: Bassoon is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors (1).
• Certificate of Analysis: 1 µg/ml of SPC-198 was sufficient for detection of Bassoon in 10 µg of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
• Species Reactivity Full Name: Human | Mouse | Rat