DESCRIPTION

• Mw: 46.3 kDa
• Tag: Myc-DDK
• Accn: NM_001146152
• Type: Human Tagged ORF Clone
• Refseq: NM_001146152.1, NP_001139624.1
• Status: 3 Weeks*
• Symbol: CYP51A1
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• Category: cDNA Clones
• Locus Id: 1595
• Orf Size: 1212 bp
• Synonyms: CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1
• Refseq Orf: 1215 bp
• Uniprot Id: Q16850
• Refseq Size: 2934 bp
• Availability: 3 Weeks
• Cytogenetics: 7q21.2
• Gene Summary: This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) The ORF insert of this clone is exactly the same as(RC228881).
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Chloramphenicol (34 ug/mL)
• Protein Families: Druggable Genome, P450, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Puromycin