Extract-EZ L1, Cytosol and Mitochondria Protein Extraction Kit: Designed to facilitate the study of apoptotic and signal transduction pathways, the kit provides unique formulations of buffers and reagents that enable isolation of highly enriched mitochondrial and cytosolic fractions from various mammalian cell lines, including both apoptotic and non-apoptotic cells. The fractionation procedure is simple and easy to perform. No ultracentrifugations are required and no toxic chemicals are involved. The enriched fractions can be used to study translocation of a variety of proteins between the mitochondrial and cytosolic fractions by Western blot analysis, ELISA, 2D electrophoresis or other assays. After lysis, different cellular compartments are separated by centrifugation of the cellular lysate at different speeds to isolate, successively, the nuclei, mitochondria and cytosol. The kit is sufficient for 50X107 cells or 50x200mg of tissue samples.