DESCRIPTION

• Notes: The AMC standard is included so the researcher can quatitatively determine the specific activity of the enzyme using the AMC standard as a measure of how much AMC substrate was cleaved to release free AMC.
• Shiptemp: -80°C (dry ice)
• Synonyms: Dipeptidylpeptidase 3
• Warnings: Avoid freeze/thaw cycles.
• Category: Protease/Assay Kit
• References: 1. Gevaert,K. et al., Proteomics 5 (14), 3589- 3599 (2005). 2. Abramic,M. et al., Int. J. Biochem. Cell Biol. 36 (3), 434-446 (2004).
• Description: The Fluorogenic DPP3 Assay Kit is designed to measure DPP3 activity using purified DPP3 for screening or profiling applications. The key to the Fluorogenic DPP3 Assay Kit is the specific, fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for DPP3 reactions; no time-consuming washing steps are required. The fluorometric substrate is incubated with a sample containing DPP3 enzyme to produce a fluorophore that can then be measured using a fluorescence reader.
• Methodology: Fluorogenic
• Supplied As: Supplied in a convenient 96-well format, with purified DPP3 enzyme, DPP substrate, and DPP assay buffer for 100 enzyme reactions.
• Unspsc Code: 41105331
• Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
• Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: At least 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Protease
• Instructions for Use: See assay kit datasheet for detailed protocol.