DESCRIPTION

• Aa: 2-end
• Mw: 66 kDa
• Tags: N-terminal FLAG-tag
• Format: Aqueous buffer solution
• Genbank: NM_199141
• Species: Human
• Uniprot: Q86X55
• Shiptemp: -80°C (dry ice)
• Synonyms: PRMT4, CARM1, Coactivator-Associated Arginine Methyltransferase 1
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Enzymes
• Background: CARM1 (PRMT4) methylates (mono- and asymmetric dimethylation) the arginine residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. It is recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at Arg17 (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), resulting in transcription activation via chromatin remodeling. CARM also methylates arginines in several other proteins, including EP300/P300 at Arg-2142, Arg-580, and Arg-604. CARM1 acts as coactivator for PPARG, and seems to be involved in p53/TP53 transcriptional activation.
• References: 1. Li H. et al., J. Biol. Chem. 277 (47), 44623-44630 (2002). 2. Frietze S. et al., Cancer Res. 68 (1), 301-306 (2008).
• Description: Human PRMT4 (CARM1), GenBank Accession No. NM_199141, a.a. 2-end, with N-terminal FLAG-tag, MW=66 kDa, expressed in FreeStyle 293-F cells.
• Formulation: 40 mM Tris-HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM DTT, 20% glycerol, and 80 ng/µl FLAG peptide
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Host Species: human
• Product Type: Protein
• Host Cell Line: FreeStyle 293-F cells
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 50 µl reaction mix (1x HMT Assay Buffer 5, BPS catalog # 52191 and 5-1000 ng methyltransferase PRMT4) is added to wells coated with the substrate on a Thermo HBC Neutravidin black plate. Incubate at room temperature for 1 hr. Add antibody against methylated R3 residue of histone H4, incubate 1 hr. Add secondary HRP-labeled antibody and incubate 30 min. Finally, add HRP chemiluminescent substrates and read luminesence.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Methyltransferase