SEK10943
Human IL13RA1 / CD213a1 ELISA Pair Set
Sino Biological
Image
DESCRIPTION
The human IL13RA1 / CD213a1 ELISA Pair Set is for the quantitative determinationof human IL13RA1.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for IL13RA1 /CD213a1 coated on a 96-well plate. Standards and samples are added to thewells, and any IL13RA1 / CD213a1 present binds to the immobilized antibody. Thewells are washed and a horseradish peroxidase conjugated mouse anti-IL13RA1monoclonal antibody is then added, producing an antibody-antigen-antibody"sandwich". The wells are again washed and TMB substrate solution is loaded,which produces color in proportion to the amount of IL13RA1 present in thesample. To end the enzyme reaction, the stop solution is added and absorbancesof the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 80 ng of recombinant IL13RA1. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A six-point standard curve usi ng 2-fold serial dilutions in sample dilution buffer, and a high standard of 2000 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 31.25-2000
- Specificity: Human IL13RA1 / CD213a1
- Capture Antibody: 0.5 mg/mL of mouse anti-IL13RA1 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 10943-MM03)
- Detection Antibody: 0.5 mg/mL of mouse anti-IL13RA1 monoclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 1.0 μg/mL in detection antibody dilution buffer before use. (Catalog: # 10943-MM07)