DESCRIPTION

• Aa: 518-end
• Mw: 78 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: AY302468
• Species: Human
• Uniprot: Q8WUI4
• Shiptemp: -80°C (dry ice)
• Synonyms: histone deacetylase 7, HDAC-7
• Warnings: Avoid freeze/thaw cycles.
• Category: Deacetylase/Enzymes
• References: 1. Chakraborty S. (2006) J.Biol.Chem. 281(46):35070-80. 2. Wolfgang, F. (2001) J. Biol. Chem. 276(38): 35826-35835. Application References: 1. Abdel-Atty, M.M., et al. Bioorg Chem. 2014 Dec;57:65-82. doi: 10.1016/j.bioorg.2014.08.006. Design, synthesis, 3D pharmacophore, QSAR, and docking studies of carboxylic acid derivatives as Histone Deacetylase inhibitors and cytotoxic agents (2014) 2. Wang, C., et al. J Med Chem. 2014 Oct 9;57(19):7999-8009. doi: 10.1021/jm500872p. In Vivo Imaging of Histone Deacetylases (HDACs) in the Central Nervous System and Major Peripheral Organs (2014) 3. Schroeder, F.A., et al. PLoS One . 2013 Aug 14;8(8):e71323. doi: 10.1371/journal.pone.0071323. A selective HDAC 1/2 inhibitor modulates chromatin and gene expression in brain and alters mouse behavior in two mood-related tests (2013) 4. Fass, D.M., et al. Neuropharmacology. 2013 Jan; 64: 81–96. Crebinostat: a novel cognitive enhancer that inhibits histone deacetylase activity and modulates chromatin-mediated neuroplasticity (2013) 5. FDG-PET imaging reveals local brain glucose utilization is altered by class I histone deacetylase inhibitors (2013)
• Description: Human HDAC7 , GenBank Accession No. AY302468, a.a. 518-end, with N-terminal GST-tag, MW= 78 kDa, expressed in baculovirus expression system.
• Formulation: 45 mM Tris-HCl, pH 8.0, 124 mM NaCl, 2.4 mM KCl, 20 mM glutathione, and 10% glycerol.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. 
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Unit Definition: One U =1 pmol of acetyl group removed/min/µg of enzyme
• Assay Conditions: 25 mM Tris HCl, pH 8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 20 µM BPS HDAC class 2a substrate (Catalog #50040), and HDAC7 (0.16 - 10 ng). Incubation condition: 30 min at 37°C, followed by_x000D_ HDAC developer (Catalog #50030) for 15 min at room temperature. Fluorescence intensity is measured at ex360/em460.
• Related Products: 50067
• Specific Activity: ≥15,000 pmol/min/µg.
• Storage Stability: At least 6 months at -80°C.
• Scientific Category: Deacetylase