CD0-H82E3-20ug CD0-H82E3-100ug
Biotinylated Human CD20 / MS4A1 Full Length Protein, His,Avitag™ (Nanodisc)
ACROBiosystems
DESCRIPTION
B-lymphocyte antigen CD20 is also known as B-lymphocyte surface antigen B1, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1 and MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. CD20 is expressed on all stages of B cell development except the first and last; it is present from late pro-B cells through memory cells, but not on either early pro-B cells or plasma blasts and plasma cells. It is found on B-cell lymphomas, hairy cell leukemia, B-cell chronic lymphocytic leukemia, and melanoma cancer stem cells. The protein has no known natural ligand and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. CD20 / MS4A1 is the target of the monoclonal antibodies (mAb) rituximab, Ibritumomab tiuxetan, and tositumomab, which are all active agents in the treatment of all B cell lymphomas and leukemias. Defects in CD20 / MS4A1 are the cause of immunodeficiency common variable type 5 (CVID5); also called antibody deficiency due to CD20 defect. CVID5 is a primary immunodeficiency characterized by antibody deficiency, hypogammaglobulinemia, recurrent bacterial infections and an inability to mount an antibody response to antigen.
DETAILS
- Tag: C-12×His & C-Avi
- Host: HEK293
- Size: 100ug
- Buffer: 20 mM HEPES, 150 mM NaCl, pH7.5
- Format: Liquid
- Purity: 90%
- Source: Biotinylated Human CD20 Full Length, His,Avitag (CD0-H82E3) is expressed from human 293 cells (HEK293). It contains AA Met 1 - Pro 297 (Accession # P11836-1).
- Storage: -70℃
- Category: MABSol® Biotin Labeled Proteins
- Molecule: CD20
- Accession: NP_068769.2
- Conjugate: Biotin-labeled
- Stability: ● The product MUST be stored at -70°C or lower upon receipt; ● -70°C for 3 months under sterile conditions.
- Exp Region: Met 1 - Pro 297
- Swiss-prot: P11836-1
- Package Size: 100ug*1
- Characteristics: The CD20 carries a polyhistidine tag at the C-terminus followed by an Avi tag with calculated MW of 36.9 kDa and migrates as 43 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. The membrane scaffold protein (MSP1D1) has calculated MW of 24.7 kDa, and it migrates as 25 kDa under reducing (R) condition (SDS-PAGE).
- Endotoxin Level: 1.0 EU per μg
- Molecular Weight: 36.9 kDa
- Shipping Condition: Dry ice
- Species Reactivity: Human
- Extra Shippinghandling Fee: Please see 'Shipping-and-Payments' sheet. Website: https://www.acrobiosystems.com/A48-Shipping-and-Payments.html