DESCRIPTION

• Uniprot: P29965
• Shiptemp: -80°C (dry ice)
• Synonyms: T cell antigen Gp39, TNF related activation protein, TRAP, tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, CD40, CD40 Molecule
• Warnings: Avoid freeze/thaw cycles.
• Category: Immunotherapy/Assay Kit
• Description: The CD40:CD40L[Biotinylated] Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of CD40:CD40L signaling. This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of biotin-labeled CD40 by streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, CD40L is coated on a 96-well plate. Next, CD40 is incubated with CD40L on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, which can be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: This kit comes in a convenient 96-well format, with biotin-labeled CD40, purified CD40L, streptavidin-labeled HRP, and assay buffer for 100 binding reactions.
• Unspsc Code: 41121811
• Unspsc Name: Laboratory microchemistry kits
• Applications: This kit is useful for screening for inhibitors of CD40L binding to CD40.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Related Products: 71174, 71191, 60626, 71257, 79102, 79572, 79512
• Storage Stability: Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Immunotherapy
• Instructions for Use: See assay kit datasheet for detailed protocol.