Human Cathepsin B / CTSB Protein, His Tag (active enzyme, MALS verified)

DESCRIPTION

Cathepsin B (CTSB) is also known as APP secretase (APPS) and CPSB, is an enzymatic protein belonging to the peptidase C1 family. Cathepsin B / CTSB is synthesized as a preproenzyme. Following removal of the signal peptide, the inactive proenzyme undergoes further modifications including removal of the pro region to result in the active enzyme. The catalytic activity of Cathepsin B / APPS contains: Hydrolysis of proteins with broad specificity for peptide bonds; Preferentially cleaves -Arg-Arg-|-Xaa bonds in small molecule substrates (thus differing from cathepsin L); In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. As a thiol protease, cathepsin B / CPSB is believed to participate in intracellular degradation and turnover of proteins and has also been implicated in tumor invasion and metastasis. Overexpression of cathepsin B has been associated with esophageal adenocarcinoma and other tumors.

DETAILS

Tag: C-6×His
Host: HEK293
Size: 50ug
Buffer: 50 mM Tris, 150 mM NaCl, pH8.0
Format: Powder
Purity: 95%
Source: Human Cathepsin B, His Tag (CTB-H5222) is expressed from human 293 cells (HEK293). It contains AA Arg 18 - Ile 339 (Accession # P07858-1 (L26V)).
Storage: -20℃
Category: Enzymes
Molecule: Cathepsin B
Accession: NP_001899.1
Conjugate: Unconjugated
Stability: ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Exp Region: Arg 18 - Ile 339
Swiss-prot: P07858-1 (L26V)
Package Size: 50ug*1
Characteristics: This protein carries a polyhistidine tag at the C-terminus. The Human Cathepsin B will be further processed into mature form (Leu 80-Ile 339). The protein has a calculated MW of 36.7 kDa (pro-form) and 29.5 kDa (mature-form). The protein migrates as 42-50 kDa and 34 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Endotoxin Level: 1.0 EU per μg
Molecular Weight: 36.7 kDa & 29.5 kDa
Shipping Condition: RT
Species Reactivity: Human
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