Anti-p53 (Phospho-Ser392) Monoclonal Antibody (Clone:FP3.2 [FPS392])

DESCRIPTION

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

DETAILS

Gene: TP53
App Id: WB
Antigen: p53 (Phospho-Ser392)
Gene Id: 7157
Isotype: Mouse IgG1
Format Id: Purified
Clone Name: FP3.2 [FPS392]
Uniprot Id: P04637
Host Animal: Mouse
Clonality Id: Monoclonal
Purification: Purified by protein-A affinity chromatography
Reactivity Id: Human
Alternative Name: TP53||P53
Application Note: Immunohistochemistry (paraffin sections): Standard ABC technique (DAB+), pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0, recommended dilution: 10µg/ml, incubation: 1 hour at RT; or overnight at 4°C, positive tissue: breast carcinoma with high level of wild-type p53. Western blotting: recommended dilution: 1µg/ml.
Storage Condition: Store at 2-8°C. Do not freeze.
Immunogen Information: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.