DESCRIPTION

• Mw: 14.6 kDa
• Tag: Myc-DDK
• Accn: NM_212511
• Type: Rat Tagged ORF Clone
• Refseq: NM_212511.2, NP_997676.1
• Status: 4 Weeks*
• Symbol: Rpl35
• Vector: pCMV6-Entry
• Category: cDNA Clones
• Locus Id: 296709
• Orf Size: 369 bp
• Synonyms: MGC72958
• Refseq Orf: 372 bp
• Uniprot Id: P17078
• Refseq Size: 476 bp
• Availability: 4 Weeks
• Cytogenetics: 3q12
• Gene Summary: mouse homolog is Rpl35, a component of the 60S ribosomal protein complex; may be a structural component of the ribosome; may be involved in protein biosynthesis [RGD, Feb 2006]
• Sequence Data: ORF Nucleotide Sequence ( show hide ) >RR204964 representing NM_212511 Red =Cloning site Blue=ORF Green =Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGC C ATGGCCAAGATTAAGGCTCGGGACCTGCGCGGCAAGAAGAAGGAGGAGCTGTTGAAACAACTGGATGATC TGAAGGTGGAACTGTCCCAGCTTCGCGTGGCCAAAGTGACAGGCGGCGCCGCGTCCAAGCTCTCCAAGAT ACGAGTCGTACGCAAATCCATCGCCCGTGTCCTCACTGTCATTAATCAGACTCAAAAGGAAAACCTCAGG AAATTCTACAAGGGAAAGAAGTACAAGCCCCTGGACCTGCGACCCAAGAAGACAAGAGCCATGCGCCGCC GGCTCACCAAGCATGAAGAGAAGCTGAAGACCAAGAAGCAGCAGCGGAAGGAGCGGCTGTACCCACTGCG CAAGTACGCAGTCAAGGCC ACGCGT ACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAG GTTTAA Protein Sequence ( show hide ) >RR204964 representing NM_212511 Red =Cloning site Green =Tags(s) MAKIKARDLRGKKKEELLKQLDDLKVELSQLRVAKVTGGAASKLSKIRVVRKSIARVLTVINQTQKENLR KFYKGKKYKPLDLRPKKTRAMRRRLTKHEEKLKTKKQQRKERLYPLRKYAVKA TR TRPLEQKLISEEDLAANDILDYKDDDDK V
• Oti Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Oti Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Reconstitution: 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• E Coli Selection: Kanamycin (25 ug/mL)
• Restriction Sites: SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Mammalian Cell Selection: Neomycin