DESCRIPTION

• Notes: To generate the AP1 eGFP reporter stable cell line, remove the growth medium 48 hours after transduction and replace it with fresh growth medium containing the appropriate amount of G418 for antibiotic selection of transduced cells. For information about determining the appropriate concentration of selection antibiotic, visit https://bpsbioscience.com/cell-line-faq in our Resources section (Q: what is a kill curve?) Biosafety: The lentiviruses are produced with the third generation SIN (self-inactivation) lentivector which ensures self-inactivation of the lentiviral construct after transduction and integration into the genomic DNA of the target cells. None of the HIV genes (gag, pol, rev) will be expressed in the transduced cells, as they are expressed from packaging plasmids lacking the packing signal and are not present in the lentivirus particle. Although the pseudotyped lentiviruses are replication-incompetent, they require the use of a Biosafety Level 2 facility. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: Visit bpsbioscience.com/lentivirus-faq for detailed troubleshooting instructions. For all further questions, please email support@bpsbioscience.com.
• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles
• Category: Cell Signaling Pathway/Lentivirus
• Background: The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun, c-Fos, ATF (Activating transcription factor), and JDP (Jun dimerization protein). These transcription factors form heterodimers termed AP1, which bind to AP1 response elements in the promoters of target genes and induce their transcription.
• Description: The Activator protein 1 (AP1) eGFP Reporter Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an eGFP gene driven by an AP1 response element located upstream of the minimal TATA promoter (Figure 1). After transduction, activation of the JNK signaling pathway and AP1-mediated activity in the target cells can be monitored by eGFP expression. Figure 1. Schematic of the lenti-vector used to generate the AP1-eGFP Reporter Lentivirus.
• Formulation: The lentivirus particles were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
• Supplied As: Two vials (500 µl x 2) of lentivirus at a titer >107 TU/ml. The titer will vary with each lot; the exact value will be provided with each shipment.
• Unspsc Code: 41106621
• Unspsc Name: Virus mediated expression vectors or kits
• Applications: Screen for activators or inhibitors of the JNK signaling pathway in the transduced target cells. Generate an AP1 eGFP Reporter stable cell line (geneticin-resistant).
• Product Type: Lentivirus
• Biosafety Level: BSL-2
• Related Products: 60612, 60405, 79617, 79665
• Storage Stability: Lentiviruses are shipped with dry ice. For long-term storage, it is recommended to store the lentiviruses at -80°C. 
• Scientific Category: Cell Signaling Pathway