APC-105_25 mcl APC-105_50 mcl APC-105_0.2 ml APC-105-CF_0.2 ml
Scientist.com Supplier
Anti-Kir6.1 (KCNJ8) Antibody
Alomone Labs
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DESCRIPTION:
Alomone Labs is pleased to offer a highly specific antibody directed against an epitope of rat Kir6.1. Anti-Kir6.1 (KCNJ8) Antibody (#APC-105) can be used in western blot, immunoprecipitation and immunohistochemistry applications. It has been designed to recognize Kir6.1 from human, rat and mouse samples. The antibody is specific for Kir6.1 and does not cross react with Kir6.2.
DETAILS
- Form: Lyophilized powder. Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4.
- Host: Rabbit
- Clone: NA
- Label: Unconjugated
- Purity: Affinity purified on immobilized antigen.
- Target: ATP-sensitive inward rectifier potassium channel 8, uKATP-1
- Comment: Contact Alomone Labs for technical support and product customization
- Gene Id: 25472
- Isotype: Rabbit IgG
- Homology: Mouse, human, pig, rabbit - 15/16 amino acid residues identical
- Is Toxin: No
- Sequence: (C)KRNSMRRNNSMRRSN, corresponding to amino acid residues 382-396 of rat Kir6.1
- Synonyms: ATP-sensitive inward rectifier potassium channel 8, uKATP-1
- Accession: Q63664
- Clonality: Polyclonal
- Lead Time: 1-2 Business Days
- Reactivity: Human|Rat|Mouse
- Formulation: PBS pH7.4
- Applications: IF|IHC|IP|WB
- Ko Validated: yes
- Preservative: No Preservative
- Reconstitution: 0.2 ml double distilled water (DDW).
- Blocking Peptide: BLP-PC105
- Negative Control: BLP-PC105
- Positive Control: NA
- Cited Application: IHC
- Immunogen Location: Intracellular, C-terminal domain
- Peptide Confirmation: Confirmed by amino acid analysis and mass spectrometry
- Shipping And Storage: Shipped at room temperature. Product as supplied can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C
- Immunogen Source Species: Rat
- Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
- Antigen Preadsorption Control: 1 µg peptide per 1 µg antibody
- Applications May Also Work In: IF|IHC|IP|WB
- Storage Before Reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C
- Product Page Scientific Background: Kir6.1 is a member of the inward rectifier K+ channels (Kir channels), a large family of voltage-independent K+ channels largely involved in stabilization of the membrane resting potential and in K+ transport across membranes.Kir6.1, like its close relative Kir6.2, is highly sensitive to inhibition by intracellular ATP. Closure of the channel leads to membrane depolarization thereby coupling intracellular metabolism to cellular excitability.Kir6.1 presents the common topology of the inward-rectifier superfamily: two transmembrane domains flanking a highly conserved pore region with the N- and C-termini located intracellularly.The functional ATP sensitive channel (KATP) is composed of octamers of four Kir6.x subunits and four members of the sulfonylurea receptor family SUR1, SUR2A and SUR2B.The channel's tissue distribution is relatively broad with expression detected in heart, brain, and smooth muscle.
- Standard Quality Control Of Each Lot: Western blot analysis
- Application Dilutions Western Blot Wb: 1:200
- Antibody Concentration After Reconstitution: 1 mg/ml
- Application Dilutions Immunohistochemistry Paraffin Embedded Sections Ihc: 1:50