80569
PARP1 Chemiluminescent Assay Kit (384-well)
BPS Bioscience
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DESCRIPTION
The PARP1 Chemiluminescent Activity Assay Kit is designed to measure
PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent
addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent
Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for
PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1
biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme.
Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to
produce chemiluminescence that can then be measured using a chemiluminescence reader.
DETAILS
- Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: 1x PBS buffer PBST buffer (1x PBS, containing 0.05% Tween-20) Luminometer or fluorescent microplate reader capable of reading chemiluminescence Adjustable micropipettor and sterile tips Rotating or rocker platform
- Species: Human
- Shiptemp: -80°C (dry ice)
- Synonyms: poly (ADP-ribose) polymerase 1, poly(ADP-ribosyl)transferase, ADPRT, PARP1
- Warnings: Avoid freeze/thaw cycles.
- Category: PARP/Assay Kit
- References: Brown JA, Marala RB. J. Pharmacol. Toxicol. Methods 2002 47:137-41. Application Reference(s): Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis (2013)
- Description: The PARP1 Chemiluminescent Activity Assay Kit is designed to measure PARP1 activity for screening and profiling applications. PARP1 is known to catalyze the NADdependent addition of poly (ADP-ribose) to histones. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with an assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
- Methodology: Chemiluminescent
- Supplied As: The PARP1 assay kit comes in a convenient 384-well format, with purified PARP1 enzyme, histone mixture, activated DNA, and PARP1 assay buffer for 400 enzyme reactions.
- Unspsc Code: 41105331
- Unspsc Name: Protein chemifluorescent detection reagents or kits or substrates
- Applications: Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 80551, 80501, 80580, 78317
- Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
- Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: PARP
- Instructions for Use: See assay kit datasheet for detailed protocol.