DESCRIPTION

• Shiptemp: -80°C (dry ice)
• Warnings: Avoid freeze/thaw cycles.
• Category: Methyltransferase/Assay Kit
• References: Svedruzic, Z.M. Curr Med Chem. 2008; 15(1) :92-106.
• Description: The DNMT Universal Assay Kit is designed to measure DNMT activity for screening and profiling applications. The key to the DNMT Universal Assay Kit is a highly specific antibody that recognizes 5-methylcytosine on the substrate. With this kit, only three simple steps on a microtiter plate are required for detection of DNMT activity. First, S-adenosylmethionine is incubated with a sample containing assay buffer and DNMT for two hours. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can be measured using a chemiluminescence reader.
• Methodology: Chemiluminescent
• Supplied As: Kit comes with a 96-well plate precoated with DNMT substrate, an antibody against 5-methylcytosine, a secondary HRP-labeled antibody, S-adenosylmethionine, DNMT assay buffer, and purified DNMT1, DNMT3A/3L and DNMT3B/3L for 100 reactions.
• Unspsc Code: 41105332
• Unspsc Name: Protein chemiluminescent detection reagents or kits or substrates
• Applications: Useful for studying enzyme kinetics and HTS applications.
• Product Type: Assay Kit
• Biosafety Level: Not applicable (BSL-1)
• Contraindications: DMSO >1%, strong acids or bases, ionic detergents, high salt
• Storage Stability: 12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
• Scientific Category: Methyltransferase
• Instructions for Use: See assay kit datasheet for detailed protocol.