Anti-α1-Catenin (N-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: CTNNA1
Host: Rabbit
Rrid: AB_2261287
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: α1-Catenin (N-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: alphaE-catenin, catenin alpha1, catenin
Clonality: Rabbit Polyclonal
Immunogen: α1-Catenin synthetic peptide (coupled to KLH) corresponding to amino acid residues from the N-terminal region of human α1-Catenin. This peptide sequence is highly conserved in rat and mouse α1-Catenin, and has some homology to α2-Catenin or α3-Catenin.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects a 102 kDa* protein corresponding to the molecular mass of α1-Catenin on SDS-PAGE immunoblots of rat PC12 and mouse SYF cells.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 102
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Chicken, Human, Mouse, and Rat
Target Description: α-catenins are cadherin interacting proteins with homology to vinculin. Three α-catenin genes have been described including α1-catenin (αE-Catenin), α2-catenin (αN-catenin), and α3-catenin (αT-catenin). α1-catenin has 81% homology with α2-catenin and 60% homology with α3-catenin. These α-catenin isoforms may have similar roles since each binds cadherins. However, their expression patterns are both overlapping and distinct. α1-catenin was identified in epithelial cells, and is expressed in various cell types. α2-catenin is enriched in the nervous system, and α3-catenin is expressed highest in testis and heart. Phosphorylation may regulate the activity of α1-catenin, since tyrosine phosphorylation of Tyr-148 occurs during intercellular adhesion. This site is dephosphorylated by SHP2, which inhibits α1-catenin binding to β-catenin and translocation to the plasma membrane. Phosphorylation of α1-catenin at Tyr-148 may be important for inhibition of cell transformation, and dephosphorylation of this site may be important during SHP2-mediated cell transformation.
Uniprot Number Immunogen Species: P35221