DESCRIPTION

• Mw: 130 kDa
• Form: Lyophilized
• Cas No: 93928-24-6
• Origin: Native protein isolated from mouse submaxillary glands.
• Purity: ≥98% (HPLC)
• Source: Natural protein
• Target: p75NTR, TrkA receptors
• Comment: Contact Alomone Labs for technical support and product customization
• Gene Id: NGFR, NTRK1
• Activity: NGF is purified in three forms: the 7S, 2.5S and β. The 7S 130 kDa form occurs naturally in mouse submaxillary glands and is a multimeric protein composed of two α, one β and two γ subunits. The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical chains held together by hydrophobic interactions. The α chain stabilizes the 7S complex and binds two zinc ions per 7S complex as a cofactor at the β-γ interface. The γ chain is an arginine specific protease, it may also have plasminongen activator activity as well as mitogenic activity for chick embryo fibroblasts1,2.
• Is Toxin: No
• Synonyms: Nerve Growth Factor 7S
• Lead Time: 1-2 Business Days
• Solubility: Centrifuge the vial before adding solvent (10,000 x g for 5 minutes) to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Tap the vial to aid in dissolving the lyophilized product. Tilt and gently roll the liquid over the walls of the vial. Avoid vigorous vortexing. Light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeat freeze-thawing may result in loss of activity.
• Formulation: Lyophilized in 0.2% Acetic acid. May contain acetate as a residual counter ion.
• Bioassay Tested: yes
• Cited Application: Electrophysiology
• Storage of Solutions: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
• Effective Concentration: EC50 = 0.2 nM
• Storage After Reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
• Reconstitution and Solubility: Centrifuge the vial (10,000 × g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing; light vortexing for up to 3 seconds is acceptable if needed. For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in sterile water at a concentration of at least 0.1 mg/mL. Divide the stock solution into small aliquots and store at -20°C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. It is recommended to prepare fresh solutions in working buffers just before use. Repeated freeze-thaw cycles may result in loss of activity.
• Storage Before Reconstitution: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store the product at -20°C. Protect from moisture.
• Product Page - Scientific Background: The neurotrophins ("neuro" means nerve and "trophe" means nutrient) are a family of soluble, basic growth factors which regulate neuronal development, maintenance, survival and death in the CNS and PNS.1NGF, the first member of the family to be discovered, was originally purified as a factor able to support survival of sympathetic and sensory spinal neurons in culture.2 It is synthesized and secreted by sympathetic and sensory target organs and provides trophic support to neurons as they reach their final target.3Neurotrophin secretion increases in the nervous system following injury. Schwann cells, fibroblasts, and activated mast cells normally synthesize NGF constitutively, however direct trauma and induction of cytokines combine to increase neurotrophin production in these cells after injury.4NGF is purified in three forms: the 7S, 2.5S and β. The 7S (130 kDa) form occurs naturally in mouse submaxillary glands, and is a multimeric protein composed of two α, one β and two γ subunits. The name is derived from its sedimentation co-efficient, 7S.The biologically active subunit is the β, which is a 26 kDa dimer composed of two identical 120 amino acid chains held together by hydrophobic interactions.5 The α chain stabilizes the 7S complex and binds two zinc ions per 7S complex as a cofactor at the β-γ interface. The γ chain is an arginine specific protease, it may also have plasminogen activator activity as well as mitogenic activity for chick embryo fibroblasts.6 The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process.7The structural hallmark of all the neurotrophins is the characteristic arrangement of the disulfide bridges known as the cysteine knot, which has been found in other growth factors such as Platelet-derived growth factor.8 There is a 95.8% homology between the rat and mouse forms, and a 85% homology between the human and mouse.NGF has been shown to regulate neuronal survival, development function and plasticity.9 Recently, involvement of NGF in processes not involving neuronal cells has been shown, such as asthma,10 psoriasis11 and wound healing.12 The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins.13