79981-1 79981-2
Spike (SARS-CoV-2) Pseudotyped Lentivirus (eGFP Reporter)
BPS Bioscience
DESCRIPTION
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection.
The SARS-CoV-2 Spike Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions also contain the enhanced GFP gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be conveniently determined via eGFP activity. The SARS-CoV-2 Spike pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility.
DETAILS
- Genbank: QHD43416.1
- Shiptemp: -80°C (dry ice)
- Synonyms: covid, covid-19, covid19, coronavirus, spike SARS lentivirus, Spike SARS-CoV-2 GFP reporter, Spike reporter
- Warnings: Avoid freeze/thaw cycles.
- Category: Coronavirus/Lentivirus
- Description: The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As the first step of the viral replication, the virus attaches to the host cell surface before entering the cell. The viral Spike protein recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARS-CoV-2 and ACE2 may offer protection against the viral infection. The SARS-CoV-2 Spike Pseudotyped Lentivirus were produced with SARS-CoV-2 Spike (Genbank Accession #QHD43416.1) as the envelope glycoproteins instead of the commonly used VSV-G. These pseudovirions also contain the enhanced GFP gene driven by a CMV promoter, therefore, the spike-mediated cell entry can be conveniently determined via eGFP activity. The SARS-CoV-2 Spike pseudotyped lentivirus can be used to measure the activity of neutralizing antibody against SARS-CoV-2 in a Biosafety Level 2 facility.
- Formulation: The lentiviruses were produced from HEK293T cells in medium containing 90% DMEM + 10% FBS.
- Supplied As: The titer will vary with each lot; the exact value is provided with each shipment.
- Unspsc Code: 41106621
- Unspsc Name: Virus mediated expression vectors or kits
- Applications: 1. Study the mechanism of viral transduction.2. Screening for neutralizing antibodies for SARS-CoV-2 Spike and ACE2.
- Product Type: Lentivirus
- Biosafety Level: BSL-2
- Related Products: 60187, 79942, 79951
- Storage Stability: Lentiviruses are shipped with dry ice. For long term storage, it is recommended to store the virus at -80°C. Avoid repeated freeze-thaw cycles. Titers can drop significantly with each freeze-thaw cycle.
- Scientific Category: Coronavirus
- Instructions for Use: See assay protocol for detailed instructions.