SEK50403
Mouse CD5 ELISA Pair Set
Sino Biological
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DESCRIPTION
The Mouse CD5 ELISA Pair Set is for the quantitative determination of Mouse CD5.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
The Sino Biological ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for CD5 coated on a 96-well plate. Standards and samples are added to the wells, and any CD5 present binds to the immobilized antibody. The wells are washed and a horseradish peroxidase conjugated rabbit anti-CD5 polyclonal antibody is then added, producing an antibody-antigen-antibody “sandwich”. The wells are again washed and TMB substrate solution is loaded, which produces color in proportion to the amount of CD5 present in the sample. To end the enzyme reaction, the stop solution is added and absorbances of the microwell are read at 450 nm.
DETAILS
- Storage: Capture Antibody: Aliquot and store at -20℃ to -80℃ for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles. Detection Antibody: Store at 4℃ and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE! Standard: Store lyophilized standard at -20℃ to -80℃ for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80℃ for up to 1 month. Avoid repeated freeze-thaw cycles.
- Standard: Each vial contains 140 ng of recombinant mouse CD5. Reconstitute standard powder with 1 mL detection antibody dilution buffer. After reconstitution, store at -20℃ to -80℃ in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 800 pg/mL is recommended.
- Lead Time: 1-2 weeks
- Application: ELISA
- Assay Range: 12.5-800
- Specificity: Mouse CD5
- Capture Antibody: 1 mg/mL of rabbit anti-mouse CD5 monoclonal antibody. Dilute to a working concentration of 2.0 μg/mL in CBS before coating. (Catalog: # 50403-R101)
- Detection Antibody: 0.5 mg/mL rabbit anti-mouse CD5 polyclonal antibody conjugated to horseradish-peroxidase (HRP). Dilute to working concentration of 0.25 μg/mL in detection antibody dilution buffer before use.