DESCRIPTION

• Format: Lentiviral plasmids
• Refseq: NM_005651, NM_005651.1, NM_005651.2, NM_005651.3, BC005355, NM_005651.4
• Status: In Stock*
• Vector: pGFP-C-shLenti
• Summary: This gene encodes a heme enzyme that plays a critical role in tryptophan metabolism by catalyzing the first and rate-limiting step of the kynurenine pathway. Increased activity of the encoded protein and subsequent kynurenine production may also play a role in cancer through the suppression of antitumor immune responses, and single nucleotide polymorphisms in this gene may be associated with autism. [provided by RefSeq, Feb 2012]
• Category: RNAi
• Locus Id: 6999
• Synonyms: HYPTRP; TDO; TO; TPH2; TRPO
• Uniprot Id: P48775
• Availability: In Stock
• Shrna Design: These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service.
• Kit Components: TDO2 - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 6999). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free.
• E Coli Selection: Chloramphenicol (34 ug/ml)
• Performance Guaranteed: OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred).
• Mammalian Cell Selection: Puromycin