Anti-EphA4 (C-terminal region) Antibody

DETAILS

Form: Antigen Affinity Purified
Gene: EPHA4
Host: Rabbit
Rrid: AB_1268960
Type: Primary Antibody
Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol
Target: EphA4 (C-terminal region)
Isotype: IgG
Storage: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.
Shipping: Blue Ice
Synonyms: SEK, Eph
Clonality: Rabbit Polyclonal
Immunogen: EphA4 synthetic peptide (coupled to carrier protein) corresponds to amino acids in the C-terminal region of human EphA4. This sequence has significant homology to the conserved site in rat and mouse EphA4, and has low homology to other EphA and EphB family members.
Expiration: After date of receipt, stable for at least 1 year at -20°C.
Specificity: The antibody detects 95 and 120 kDa* bands corresponding to EphA4 in Western blots of HUVEC cells and mouse brain tissue.
Applications: WB
Conjugate Exem: Unconjugated
Physical State: Liquid
Quality Control: Western blots performed on each lot.
Usage Statement: For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
Molecular Weight: 95/120
Dilution Range Wb: 1:1000
Immunogen Species: Human
Species Reactivity: Human, Mouse, and Rat
Target Description: The Eph family of Receptor tyrosine kinases and their Ephrin ligands are important for cell positioning and morphogenesis during development. Eph receptors are classified into 10 EphA and 6 EphB receptors, which preferentially bind to the type A and type B ephrins, respectively. The EphA4 receptor can inhibit axon outgrowth and has roles in regulating axon projections during neural development. EphA4 signaling pathways require its kinase activity and involve binding and activation of Rho-GTPase guanine nucleotide-exchange factors (GEFs). EphA4 activation leads autophosphorylation of Tyr-596 and Tyr-602, and the conserved sites in EphA2 are required for binding to the GEFs, Vav2 and Vav3, and ephrin-induced cell migration. The Tyr-779 site in the kinase domain is also phosphorylated in vivo and may regulate kinase activity. Activated EphA4 leads to Src kinase phosphorylation of the GEF, ephexin-1, and this activates RhoA. Thus, EphA4 signaling involves complex tyrosine phosphorylation in its cytoplasmic region along with interaction with several GEFs.
Uniprot Number Immunogen Species: P54764