DESCRIPTION

• Aa: 1024-1306
• Mw: 58.9 kDa
• Tags: N-terminal GST-tag
• Format: Aqueous buffer solution
• Genbank: NM_002843
• Species: Human
• Uniprot: Q12913
• Shiptemp: -80°C (dry ice)
• Synonyms: DEP1, density-enhanced phosphatase-1, PTP-eta, CD148, PTPRJ
• Warnings: Avoid freeze/thaw cycles.
• Category: Protein Phosphatase/Receptor
• Background: Tyrosine phosphatase which dephosphorylates or contributes to the dephosphorylation of CTNND1, FLT3, PDGFRB, MET, RET (variant MEN2A), KDR, LYN, SRC, MAPK1, MAPK3, EGFR, TJP1, OCLN, PIK3R1 and PIK3R2. Plays a role in cell adhesion, migration, proliferation and differentiation. Positive regulator of platelet activation and thrombosis. Positive regulator of endothelial cell survival, as well as of VEGF-induced SRC and AKT activation through KDR dephosphorylation. Negative regulator of cell proliferation. Negative regulator of PDGF-stimulated cell migration through dephosphorylation of PDGFR. Negative regulator of EGFR signaling pathway via EGFR dephosphorylation. Enhances the barrier function of epithelial junctions during reassembly. Negatively regulates T-cell receptor (TCR) signaling. Upon T-cell TCR activation, it is up-regulated and excluded from the immunological synapses, while upon T-cell-antigen presenting cells (APC) disengagement, it is no longer excluded and can dephosphorylate PLCG1 and LAT to down-regulate prolongation of signaling.
• References: 1. A. Massa, et al., J Biol Chem. 2004, 279: 29004-12. 2. S. Kellie, et al., J Cell Sci. 2004, 117 :609-18.
• Description: Protein tyrosine phosphatase DEP1 (density-enhanced phosphatase-1), also known as PTPRJ, PTP-eta and CD148 (GenBank accession no. NM_U10886), a.a. 1024-1306 (catalytic domain) with N-terminal GST-tag, MW=58.9 kDa, expressed in an E. coli expression system.
• Formulation: 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, 10 mM glutathione.
• Unspsc Code: 12352202
• Unspsc Name: Proteins
• Applications: Useful for the study of enzyme kinetics and regulation, to dephosphorylate target substrates and for screening inhibitors.
• Product Type: Protein
• Biosafety Level: Not applicable (BSL-1)
• Assay Conditions: 20 mM HEPES, pH 7.2, 100 mM NaCl, 2 mM EDTA, 1 mM DTT, 1 mM pNPP and DEP1. Assay was done at 30°C for 10 minutes, and the reaction was monitored at 405 nm continuously. Specific activity was calculated based on ε pNPP= 18,000 M-1cm-1.
• Specific Activity: 268 nmole/min/µg
• Storage Stability: >12 months at -80°C
• Scientific Category: PTP/Receptor