DESCRIPTION

• Nature: Recombinant
• Purity: >95%
• Target: Alpha Synuclein N-Terminal Acetylated
• Category: Protein
• Conjugate: No Tag
• References: 1. “Genetics Home Reference: SNCA”. US National Library of Medicine. (2013). 2. Zhang L., et al. (2008) Brain Res. 1244: 40-52. 3. Alim M.A., et al. (2002) J Biol Chem. 277(3): 2112-2117. 4. Kokhan V.S., Afanasyeva M.A., Van'kin G. (2012) Behav. Brain. Res. 231(1): 226-230. 5. Spillantini M.G., et al. (1997) Nature. 388(6645): 839-840. 6. Mezey E., et al. (1998) Nat Med. 4(7): 755-757. 7. Anderson, J.P. et al. (2006) J Biol Chem. 2006;281:29739–29752. 8. Vinueza-Gavilanes, R. et al. (2020) Neurobiol Dis. 137, 2020, Article 104783
• Applications: WB | SDS-PAGE | In vivo assay | In vitro assay
• Field of Use: Not for use in humans. Not for use in diagnostics or therapeutics. For research use only.
• Protein Size: ~14.46kDa
• Purification: Ion-exchange Purified
• Concentration: Lot/batch specific. See included datasheet.
• Protein Length: Full Length
• Research Areas: Neuroscience | Neurodegeneration | Alzheimer's Disease | Tangles & Tau | Neuroscience | Neurodegeneration | Parkinson's Disease | Synuclein | Neuroscience | Neurodegeneration | Multiple System Atrophy
• Storage Buffer: PBS pH 7.4
• Alternative Names: N-acetylated Alpha synuclein monomer, N-terminal acetylated Alpha-synuclein monomer, Alpha synuclein protein monomer, Alpha synuclein monomer, Alpha-synuclein protein, Non-A beta component of AD amyloid protein, Non-A4 component of amyloid precursor protein, NACP protein, SNCA protein, NACP protein, PARK1 protein, Alpha synuclein monomers, SYN protein, Parkinson's disease familial 1 Protein, N‐terminal Ac‐αsyn
• Cite This Product: Human Recombinant Alpha Synuclein Protein (StressMarq Biosciences Inc., Victoria BC CANADA, Catalog # SPR-332B)
• Expression System: E. coli
• Species Full Name: Human
• Biological Activity: Seeds aggregation of N-acetylated alpha synuclein monomer (SPR-331) in thioflavin T assay
• Storage Temperature: -80ºC
• Shipping Temperature: Dry Ice. Shipping note: Product will be shipped separately from other products purchased in the same order.
• Cellular Localization: Cytoplasm | Membrane | Nucleus
• Scientific Background: Alpha-Synuclein (SNCA) is expressed predominantly in the brain, where it is concentrated in presynaptic nerve terminals (1). Alpha-synuclein is highly expressed in the mitochondria of the olfactory bulb, hippocampus, striatum and thalamus (2). Functionally, it has been shown to significantly interact with tubulin (3), and may serve as a potential microtubule-associated protein. It has also been found to be essential for normal development of the cognitive functions; inactivation may lead to impaired spatial learning and working memory (4). SNCA fibrillar aggregates represent the major non A-beta component of Alzheimers disease amyloid plaque, and a major component of Lewy body inclusions, and Parkinson's disease. Parkinson's disease (PD) is a common neurodegenerative disorder characterized by the progressive accumulation in selected neurons of protein inclusions containing alpha-synuclein and ubiquitin (5, 6). Alpha synuclein purified from both normal and pathological brain tissue is N-acetylated (7) and this post-translational modification affects affect alpha-synuclein stability and toxicity (8).
• Certificate of Analysis: Acetylation cofirmed via ESI mass spectrometry