78636-1 78636-2
Bald VSV Delta G (Luciferase Reporter)
BPS Bioscience
DESCRIPTION
The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.
DETAILS
- Notes: Biosafety: The infectivity of VSV Delta G is restricted to a single round of replication. The pseudovirus can be handled using Biosafety Level 2 containment practices. BPS Bioscience recommends following all local federal, state, and institutional regulations and using all appropriate safety precautions. Troubleshooting Guide: For all further questions, please email support@bpsbioscience.com.
- Species: Vesicular stomatitis virus, VSV Delta G Pseudotype
- Shiptemp: -80°C (dry ice)
- Warnings: Avoid freeze/thaw cycles.
- Category: Coronavirus/VSV
- Background: Vesicular stomatitis virus (VSV) is an enveloped, negative-stranded RNA virus that infects a wide range of animals and less frequently humans, causing mild flu-like symptoms. Its simple structure and its ability to grow in most mammalian cell types has made VSV a valuable tool to study virus entry, replication, and assembly. The glycoprotein of VSV (VSV-G), which binds to the LDL-receptor (low-density lipoprotein receptor), is responsible for the attachment and entry of VSV into a susceptible host cell. Recombinant VSV in which the glycoprotein was deleted (VSV Delta G) can accept viral envelop proteins from a variety of other viruses, allowing to generate pseudotypes that represent robust models to screen for neutralizing antibodies and other inhibitors of virus entry. The pseudoviruses can be engineered to transduce a reporter gene such as Firefly Luciferase or a fluorescent protein, so that viral entry can be monitored using luminescence or fluorescence.
- Description: The bald VSV Delta G (Luciferase Reporter) was produced without envelope glycoproteins. It contains the firefly luciferase gene as the reporter. The bald VSV Delta G (Luciferase Reporter) can serve as a negative control when studying virus entry initiated by specific interactions between virus particles and receptors.
- Formulation: The pseudoviruses were produced from HEK293T cells. They are supplied in cell culture medium containing 90% DMEM + 10% FBS.
- Supplied As: Since the virus is lacking the envelope glycoproteins and cannot transduce target cells, functional titer of this product cannot be determined.
- Unspsc Code: 41106621
- Unspsc Name: Virus mediated expression vectors or kits
- Applications: Ideal as a negative control to study transduction by any pseudotype of VSV delta G.
- Product Type: VSV
- Biosafety Level: BSL-2
- Related Products: 60187, 78637, 78635, 78634
- Storage Stability: The VSV Delta G pseudovirus is shipped with dry ice. For long-term storage, it is recommended to store the virus at -80°C.
- Scientific Category: Coronavirus