mouse monoclonal IgG1; Cortactin Antibody (H-5) is an IgG1 κ mouse monoclonal Cortactin antibody (also designated CTTN antibody, or EMS1 antibody) that detects the Cortactin protein of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA. Cortactin Antibody (H-5) is available as both the non-conjugated anti-Cortactin antibody form, as well as multiple conjugated forms of anti-Cortactin antibody, including agarose, HRP, PE, FITC and multiple Alexa Fluor® conjugates. Cortactin (also designated Ems-1) is a filamentous Actin (F-Actin) binding protein that has been shown to be a substrate for Src p60. Cortactin contains tandem 37 amino acid repeats at the amino-terminus and an SH3 domain at the carboxy-terminus. The tandem repeats appear to be necessary for F-Actin binding. Tyrosine phosphorylation of Cortactin by Src p60 results in diminished F-Actin binding to Cortactin and reduced F-Actin cross-linking activity. Cortactin has also been shown to be phosphorylated in response to FGF-1. Cortactin exhibits abundant expression in megakaryocytes and platelets, and it may play a role in the maturation of megakaryocytes.