52084
Histone H3-H4 Tetramer H3K27C Unmodified, His-tag Recombinant
BPS Bioscience
DETAILS
- Mw: 57 kDa
- Tags: N-terminal His-tag
- Format: Aqueous buffer solution
- Genbank: NM_033445, NM_003528, NM_003532, and NM_003548
- Species: Human
- Uniprot: P68431
- Shiptemp: -80°C (dry ice)
- Synonyms: H3/H4
- Warnings: Avoid freeze/thaw cycles.
- Category: Methyltransferase/Substrates
- References: 1. Simon, M.D., et al. Cell 2007; 128: 1003-1012.2. Raut, V.V., Pandey, S.M., Sainis, J.K. Ann Bot. 2011; 108(7):1235-46
- Description: Human recombinant histone tetramer consisting of 2 molecules each of histones H3 (a.a. 2-136(end)) and H4 (a.a. 2-103(end)), expressed in an E. coli expression system. Histone H3 has K27C, C97A, and C111A mutations and was alkylated to produce the lysine analog, aminoethylcysteine. GenBank Accession Nos. NM_003532, and NM_003548, respectively. Each histone protein has an N-terminal His-tag. Global MW = 57 kDa.
- Formulation: 45 mM Tris pH 7.5, 2 M NaCl, and 10% glycerol
- Unspsc Code: 12352202
- Unspsc Name: Proteins
- Applications: A perfect substrate for histone methyltransferases.
- Product Type: Protein
- Host Cell Line: E. coli
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 52064-2, 52086, 52087
- Storage Stability: At least 6 months at -80°C.
- Scientific Category: Methyltransferase
DESCRIPTION
Human recombinant histone tetramer
consisting of 2 molecules each of
histones H3 (a.a. 2-136(end)) and H4
(a.a. 2-103(end)), expressed in an E. coli
expression system. Histone H3 has
K27C, C97A, and C111A mutations and
was alkylated to produce the lysine
analog, aminoethylcysteine. GenBank
Accession Nos. NM_003532, and
NM_003548, respectively. Each histone
protein has an N-terminal His-tag.
Global MW = 57 kDa.