32632
BPTF/FALZ TR-FRET Assay Kit
BPS Bioscience
DESCRIPTION
The BPTF TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF,
also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based
assay requires no time-consuming washing steps, making it especially suitable for high
throughput screening applications. The assay procedure is straightforward and simple; a sample
containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is
incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence
reader.
DETAILS
- Notes: MATERIALS OR INSTRUMENTS REQUIRED BUT NOT SUPPLIED: Fluorescent microplate reader capable of measuring Time Resolved Fluorescence Resonance Energy Transfer (TR-FRET) Adjustable micropipettor and sterile tips
- Uniprot: Q12830
- Shiptemp: -80°C (dry ice)
- Synonyms: BPTF, FALZ
- Warnings: Avoid freeze/thaw cycles.
- Category: Bromodomain/Assay Kit
- References: Filippakopoulos, P., et al., Cell 2012; 149:214.
- Description: The BPTF TR-FRET Assay Kit is designed to measure the inhibition of the binding of BPTF, also known as FALZ, to its substrate in a homogeneous 384 reaction format. This FRET-based assay requires no time-consuming washing steps, making it especially suitable for high throughput screening applications. The assay procedure is straightforward and simple; a sample containing terbium-labeled donor, dye-labeled acceptor, BPTF, substrate, and an inhibitor is incubated for 2 hours. Then, the fluorescence intensity is measured using a fluorescence reader.
- Methodology: TR-FRET
- Supplied As: Kit comes in a convenient format with purified BPTF/FALZ, ligands, Tb donor, dye-labeled acceptor, assay buffer and microtiter plate to perform 384 reactions
- Unspsc Code: 41121811
- Unspsc Name: Laboratory microchemistry kits
- Applications: Great for screening small molecular inhibitors for drug discovery and HTS applications.
- Product Type: Assay Kit
- Biosafety Level: Not applicable (BSL-1)
- Related Products: 31131, 31134, 70040-1, 72020
- Storage Stability: At least 6 months from date of receipt when stored as directed. Be sure to store each component at the proper temperature upon arrival.
- Scientific Category: Bromodomain
- Instructions for Use: See assay kit datasheet for detailed protocol.